Anti-SOS Alexa Fluor® 647

The guanine nucleotide exchange factor Sos (son-of-sevenless) is a complex multidomain protein that activates the small GTPase Ras (H-Ras, K-Ras, N-Ras, but not functionally distinct R-Ras) in response to receptor tyrosine kinase stimulation. Nucleotide exchange activity of Sos is stimulated by allosteric Ras binding. By another (separable) guanine exchange factor domain domain Sos modulates activity of Rac/Rho GTPases. Sos thus integrates signals that affect both gene expression and cytoskeletal reorganization; the Sos-mediated Ras-activation and Rac activation differ in composition and stability of the formed complex.

Mouse, Human, Non-human primates

Peptide corresponding to amino acids THPSMHRDGPPLLENAHSS of human Sos protein.

SOS

SOS-01

ICC

0.1 mg/mL

Alexa Fluor® 647

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

*Tian X, Feig LA: Basis for signaling specificity difference between Sos and Ras-GRF guanine nucleotide exchange factors. J Biol Chem. 2001 Dec 14;276 (50) :47248-56., URL: http://www.ncbi.nlm.nih.gov/pubmed/11560935?ordina, *Silver SJ, Chen F, Doyon L, Zink AW, Rebay I: New class of Son-of-sevenless (Sos) alleles highlights the complexities of Sos function. Genesis. 2004 Aug;39 (4) :263-72., URL: https://www.ncbi.nlm.nih.gov/pubmed/15286999?ordin, *Innocenti M, Tenca P, Frittoli E, Faretta M, Tocchetti A, Di Fiore PP, Scita G: Mechanisms through which Sos-1 coordinates the activation of Ras and Rac. J Cell Biol. 2002 Jan 7;156 (1) :125-36., URL: https://www.ncbi.nlm.nih.gov/pubmed/11777939?ordin, *Freedman TS, Sondermann H, Friedland GD, Kortemme T, Bar-Sagi D, Marqusee S, Kuriyan J: A Ras-induced conformational switch in the Ras activator Son of sevenless. Proc Natl Acad Sci U S A. 2006 Nov 7;103 (45) :16692-7., URL: https://www.ncbi.nlm.nih.gov/pubmed/17075039?ordin, *Yang L, Bashaw GJ: Son of sevenless directly links the Robo receptor to rac activation to control axon repulsion at the midline. Neuron. 2006 Nov 22;52 (4) :595-607., URL: https://www.ncbi.nlm.nih.gov/pubmed/17114045?ordin

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The antibody SOS-01 reacts with human Sos, an ubiquitously expressed 150 kDa intracellular protein. The epitope sequence is highly conserved and reactivity with multiple species is expected.

Mouse IgG1