Anti-Sox2 Antilichaam

Embryonic stem cells can maintain a pluripotent state that is controlled by a set of transcription factors that include Oct-4, Sox2, and Nanog. Chromatin immunoprecipitation experiments show that Sox2 and Oct-4 bind to thousands of gene regulatory sites, many of which regulate cell pluripotency and early embryonic development. siRNA knockdown of either Sox2 or Oct-4 results in loss of pluripotency, while overexpression of Oct-4 and Sox2, along with additional transcription factors Klf4 and c-Myc, can reprogram somatic cells to a pluripotent state. Sox2 also regulates adult multipotent progenitors in various epithelial tissues, and may be important for survival and regeneration of these tissues. The activity of Sox2 may be regulated by phosphorylation and methylation. Akt1 phosphorylates Thr-118 and enhances Sox2 transcriptional activity, while Set7 can monomethylate Lys-119 leading to inhibition of Sox2 transcriptional activity, as well as Sox2 ubiquination and degradation. In addition, Sox2 Thr-128 is constituitively phoshorylated in the F9 mouse stem cell line.

Our Sox2 primary antibody from PhosphoSolutions is mouse monoclonal. It detects chicken, human, mouse, rat, and xenopus Sox2 and is protein g purified. It is great for use in ELISA, WB, ICC.

Our Sox2 primary antibody from PhosphoSolutions is mouse monoclonal. It detects chicken, human, mouse, rat, and Xenopus Sox2 and is Protein G purified. It is great for use in WB, ICC.

SRY (sex determining region Y) box-2, ANOP3, MCOPS3, SOX-2

SOX2

P48431

Chicken, Human, Mouse, Rat, and Xenopus

Clone M551 was generated from a recombinant protein corresponding to the full length sequence from human Sox2. This sequence is highly conserved in rat and mouse Sox2.

Sox2

M551

Unconjugated

ELISA, WB, ICC

Cell Markers, Neuronal Markers, Immature Neurons, Neurogenesis and Development, Oncoproteins, Transcription Regulation

Protein G Purified

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

34

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Blue Ice

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Protein G purified culture supernatant.

1:500

This antibody antibody detects a 34 kDa* protein on SDS-PAGE immunoblots of mouse F9 stem cells, and detects a full length recombinant human Sox2 protein.

1:50

Western blots performed on each lot.

Mouse

Liquid

IgG2b

Human

Quality Control: WB

AB_3068501