Anti-Sphingosine Kinase 1 (C-terminaal gebied) Antilichaam

Sphingolipids are metabolized into bioactive products that include ceramide, sphingosine, and sphingosine-1-phosphate (S1P) . Sphingosine Kinase (SK) catalyzes the phosphorylation of the lipid sphingosine, creating S1P. S1P subsequently signals through cell surface G protein-coupled receptors, as well as intracellularly, to modulate cell proliferation, survival, motility and differentiation. Two isoforms of SK have been identified, SK1 and SK2. The mRNA for both of these isoforms is widely expressed with SK1 expression highest in brain, heart, kidney, thymus, spleen and lung, while SK2 is highest in kidney and liver. SKs can be activated through growth factor, G protein-coupled, and immunoglobulin receptor signalling. SK1 has been shown to mediate cell growth, prevention of apoptosis, and cellular transformation, and is upregulated in a variety of human tumors. Regulation of SK1 may occur through ERK mediated phosphorylation of Ser-225. This phosphorylation leads to increased activity and translocation to the plasma membrane.

Our Sphingosine Kinase 1 (C-terminal region) rat rat monoclonal primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat Sphingosine Kinase 1 (C-terminal region) and is antigen affinity purified. It is great for use in ELISA, WB, ICC.

Our Sphingosine Kinase 1 (C-terminal region) rat rat monoclonal primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat Sphingosine Kinase 1 (C-terminal region) and is antigen affinity purified. It is great for use in WB, ICC.

SK1, SPHK1, SPK1

SPHK1

Q8CI15

Human, Mouse, and Rat

Clone M540 was generated from a SK1 synthetic peptide (coupled to carrier) corresponding to amino acids in the C-terminal region of mouse SK1. This sequence has high homology to human and rat SK1, and is not homologous to sequences in SK2.

Sphingosine Kinase 1 (C-terminal region)

M540

Unconjugated

ELISA, WB, ICC

Cell Signaling, G Protein Signaling, Growth Factor Signaling, Kinase Signaling, Oncoproteins

Antigen Affinity Purified

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

42

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Blue Ice

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Affinity purified rabbit serum

1:250

The antibody detects 42 kDa* full-length recombinant human and mouse SK1 proteins, as well as detects 42, 50, and 60 kDa forms of SK1 in human HeLa cells.

1:200

Western blots performed on each lot.

Rat

Liquid

N.T.

Mouse

Quality Control: WB

AB_3068500