Anti-Sphingosine Kinase 1 (Centrale regio) Antilichaam

Sphingolipids are metabolized into bioactive products that include ceramide, sphingosine, and sphingosine-1-phosphate (S1P) . Sphingosine Kinase (SK) catalyzes the phosphorylation of the lipid sphingosine, creating S1P. S1P subsequently signals through cell surface G protein-coupled receptors, as well as intracellularly, to modulate cell proliferation, survival, motility and differentiation. Two isoforms of SK have been identified, SK1 and SK2. The mRNA for both of these isoforms is widely expressed with SK1 expression highest in brain, heart, kidney, thymus, spleen and lung, while SK2 is highest in kidney and liver. SKs can be activated through growth factor, G protein-coupled, and immunoglobulin receptor signalling. SK1 has been shown to mediate cell growth, prevention of apoptosis, and cellular transformation, and is upregulated in a variety of human tumors. Regulation of SK1 may occur through ERK mediated phosphorylation of Ser-225. This phosphorylation leads to increased activity and translocation to the plasma membrane.

Our Sphingosine Kinase 1 (Central region) rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects human Sphingosine Kinase 1 (Central region) and is antigen affinity purified. It is great for use in ELISA, WB.

Our Sphingosine Kinase 1 (Central region) rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects human Sphingosine Kinase 1 (Central region) and is antigen affinity purified. It is great for use in WB.

SK1, SPHK1, SPK1

SPHK1

Q9NYA1

Human

SK1 synthetic peptide (coupled to carrier protein) corresponding to an unphosphorylated sequence of amino acids surrounding serine 225 in the central region of human SK1. This sequence has 4 amino acid differences from mouse and 5 from rat SK1, and is not homologous to sequences in SK2.

Sphingosine Kinase 1 (Central region)

Unconjugated

ELISA, WB

Cell Signaling, G Protein Signaling, Growth Factor Signaling, Kinase Signaling, Oncoproteins

Antigen Affinity Purified

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

47

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Blue Ice

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Affinity purified rabbit serum

1:1000

The antibody detects a 47 kDa* full-length recombinant human SK1 protein. In mouse, the antibody detects SK1 in isolated cardiac myocytes, but not in SK1-null mice (Tao, R. et al. (2007) Cardiovas. Res. 74:56.) .

Western blots performed on each lot.

Rabbit

Liquid

IgG

Human

Quality Control: WB

AB_2195828