Anti-SPHK1 Antibody Picoband® fluoro488 Vervoeging

SPHK1 (Sphingosine Kinase 1), is an enzyme that in humans is encoded by the SPHK1 gene. Melendez et al. (2000) mapped the SPHK1 gene to chromosome 17q25.2 based on sequence identity with ESTs mapped to this region. Kohama et al. (1998) demonstrated that recombinant mouse Sphk1 can specifically phosphorylate D-erythro-sphingosine and that D, L-threo-dihydrosphingosine and N, N-dimethylsphingosine can act as competitive inhibitors of recombinant Sphk1. Pitson et al. (2000) found that recombinant SPHK1 and endogenous SPHK1 purified from placenta had identical enzymatic characteristics, suggesting posttranslational modification does not effect functional properties.

Hexokinase-2;2.7.1.1; Hexokinase type II; HK II; Muscle form hexokinase; HK2

SPHK1

8877

Q9NYA1

Human

No cross-reactivity with other proteins.

E.coli-derived human SPHK1 recombinant protein (Position: E55-N357) .

Predominant hexokinase isozyme expressed in insulin-responsive tissues such as skeletal muscle.

Flow Cytometry

Cancer, Cancer Metabolism, Carbohydrate Metabolism, Cardiovascular, Energy Metabolism, Energy Transfer Pathways, Metabolic Signaling Pathway, Metabolic Signaling Pathways, Metabolism, Metabolism Of Carbohydrates, Pathways and Processes, Signal Transduction

Immunogen affinity purified.

Liquid

E3 ubiquitin-protein ligase that mediates ubiquitination of Delta receptors, which act as ligands of Notch proteins. Positively regulates the Delta-mediated Notch signaling by ubiquitinating the intracellular domain of Delta, leading to endocytosis of Delta receptors. Probably mediates ubiquitination and subsequent proteasomal degradation of DAPK1, thereby antagonizing anti-apoptotic effects of DAPK1 to promote TNF- induced apoptosis (By similarity) . Involved in ubiquitination of centriolar satellite CEP131, CEP290 and PCM1 proteins and hence inhibits primary cilium formation in proliferating cells. Mediates 'Lys-63'-linked polyubiquitination of TBK1, which probably participates in kinase activation.

1. Kohama, T., Olivera, A., Edsall, L., Nagiec, M. M., Dickson, R., Spiegel, S. Molecular cloning and functional characterization of murine sphingosine kinase. J. Biol. Chem. 273: 23722-23728, 1998. 2. Melendez, A. J., Carlos-Dias, E., Gosink, M., Allen, J. M., Takacs, L. Human sphingosine kinase: molecular cloning, functional characterization and tissue distribution. Gene 251: 19-26, 2000. 3. Pitson, S. M., D'Andrea, R. J., Vandeleur, L., Moretti, P. A. B., Xia, P., Gamble, J. R., Vadas, M. A., Wattenberg, B. W. Human sphingosine kinase: purification, molecular cloning and characterization of the native and recombinant enzymes. Biochem. J. 350: 429-441, 2000.

At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

102380 MW

6

Hexokinase-2

Mitochondrion outer membrane . Its hydrophobic N-terminal sequence may be involved in membrane binding. .

Hexokinase-2

Rabbit IgG

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.