Anti-Stat1 (Tyr-701), Fosfospecifiek antilichaam

The stat proteins function both as cytoplasmic signal transducers and as activators of transcription. Stat1 is expressed as two variants of 84 and 91 kDa. Stat1 proteins contain SH2 and SH3 domains, and are components of the interferon-stimulated gene factor 3 (ISGF3) complex. This complex is the primary transcription activator induced by the binding of interferon to its receptors. In response to activation by various cytokines and growth factors, stat1 subunits become phosphorylated at tyrosine 701. This leads to translocation of stat1 to the nucleus, resulting in formation of an active ISGF3 complex. Active ISGF3 modulates the transcription of the interferon-stimulated genes. Thus, phosphorylation of Tyr-701 is critical for gene expression mediated by various cytokines and growth factors.

Our Stat1 (Tyr-701) mouse monoclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat Stat1 (Tyr-701) and is protein a purified. It is great for use in ELISA, WB.

Our Stat1 (Tyr-701) mouse monoclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat Stat1 (Tyr-701) and is Protein A purified. It is great for use in WB.

Stat1a, Stat1b, ISGF3, Stat1alpha, Stat1beta

STAT1

P42224

Tyr-701

Human, Mouse, and Rat

Clone (M135) was generated from a synthetic peptide (coupled to KLH) corresponding to amino acid residues around tyrosine 701 of human Stat1. This peptide sequence has high homology to the conserved tyrosine site in rat and mouse stat1.

Stat1 (Tyr-701)

M135

Unconjugated

ELISA, WB

Cell Signaling, Oncoproteins, Transcription Regulation

Protein A Purified

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Phosphorylated

84/91

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Blue Ice

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Protein A purified culture supernatant.

1:1000

The antibody detects 84 and 91 kDa* stat1 variants on SDS-PAGE immunoblots of human A431 treated with EGF, as well as Jurkat and A431 cells treated with pervanadate. The antibody does not detect these variants in control cells.

Western blots performed on each lot.

Mouse

Liquid

IgG1

Human

Quality Control: WB

AB_2198140