Anti-Stat5 (Tyr-694), fosforspecifiek antilichaam

The stat proteins function both as cytoplasmic signal transducers and as activators of transcription. Stat5 is activated in response to a wide variety of ligands including IL-2, GM-CSF, growth hormone, and prolactin. Phosphorylation at Tyr-694 is required for Stat5A activation. Stat5 has been found to be constitutively active in some leukemic cell types. Phosphorylated Stat5 is found in some endothelial cells treated with IL-3, which suggests its involvement in angiogenesis and cell motility. Both Stat5A (Tyr-694) and Stat5B (Tyr-699) are independently regulated and activated in various cell types. For instance, both isoforms are activated in response to IFNα in B cells, but only Stat5A is phosphorylated in response to IFNα in HeLa cells.

Our Stat5 (Tyr-694) mouse monoclonal primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat Stat5 (Tyr-694) and is protein a purified. It is great for use in ELISA, WB, ICC.

Our Stat5 (Tyr-694) mouse monoclonal primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat Stat5 (Tyr-694) and is Protein A purified. It is great for use in WB, ICC.

Signal transducer and activator of transcription 5A, Stat5A

STAT5A

P42229

Tyr-694

Human, Mouse, and Rat

Clone (M148) was generated from a synthetic peptide (coupled to KLH) corresponding to amino acid residues around tyrosine 694 of human Stat5A. This peptide sequence is identical to tyrosine 699 in human Stat5B, and has high homology to the conserved tyrosine site in rat and mouse Stat5A/B.

Stat5 (Tyr-694)

M148

Unconjugated

ELISA, WB, ICC

Cell Signaling, Oncoproteins, Transcription Regulation

Protein A Purified

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

GOmAb/Phosphorylated

92

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Blue Ice

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Protein A purified culture supernatant.

1:1000

The antibody detects a 92 kDa* band corresponding to Stat5A on SDS-PAGE immunoblots of human A431 cells treated with EGF, but does not detect this band in control cells.

1:100

Western blots performed on each lot.

Mouse

Liquid

IgG1

Human

Quality Control: WB

AB_2286763