Anti-SYK Alexa Fluor® 647

Syk is a cytoplasmic protein tyrosine kinase that translocates to the plasma membrane upon B cell antigen receptor (BCR) or the high-affinity IgE receptor (FcepsilonRI) triggering, and phosphorylates downstream adaptor proteins, thereby providing docking sites for initiation of subsequent signaling pathways, such as calcium mobilization, cytoskeleton remodeling, or transcription of specific genes. Syk binds to the receptor assemblies through interactions of its pair of SH2 domains with ITAM motives of the receptor, which have been phosphorylated by Src-family kinases. These kinases also help to activate Syk by phosphorylation of its activation loop.

Human, Rat, Mouse

Recombinant fragment (aa 5-360) of human Syk.

SYK

SYK-01

IHC-P, ICC

0.1 mg/mL

Alexa Fluor® 647

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

*Osicka R, Osickova A, Hasan S, Bumba L, Cerny J, Sebo P: Bordetella adenylate cyclase toxin is a unique ligand of the integrin complement receptor 3. Elife. 2015 Dec 9;4:e10766., URL: https://www.ncbi.nlm.nih.gov/pubmed/26650353 , *Heneberg P, Lebduška P, Draberova L, Korb J, Draber P: Topography of plasma membrane microdomains and its consequences for mast cell signaling. Eur J Immunol. 2006 Oct;36 (10) :2795-806. , URL: https://www.ncbi.nlm.nih.gov/pubmed/17013982?dopt=, *Halova I, Draberova L, Draber P: A novel lipid raft-associated glycoprotein, TEC-21, activates rat basophilic leukemia cells independently of the type 1 Fc epsilon receptor. Int Immunol. 2002 Feb;14 (2) :213-23., URL: https://www.ncbi.nlm.nih.gov/pubmed/11809740?dopt=, *Arudchandran R, Brown MJ, Peirce MJ, Song JS, Zhang J, Siraganian RP, Blank U, Rivera J: The Src homology 2 domain of Vav is required for its compartmentation to the plasma membrane and activation of c-Jun NH (2) -terminal kinase 1. J Exp Med. 2000 Jan 3;191 (1) :47-60., URL: https://www.ncbi.nlm.nih.gov/pubmed/10620604?dopt=

*Parravicini V, Gadina M, Kovarova M, Odom S, Gonzalez-Espinosa C, Furumoto Y, Saitoh S, Samelson LE, O'Shea JJ, Rivera J: Fyn kinase initiates complementary signals required for IgE-dependent mast cell degranulation. Nat Immunol. 2002 Aug;3 (8) :741-8. Epub 2002 Jul 1. , URL: https://www.ncbi.nlm.nih.gov/pubmed/12089510?dopt=, *Manetz TS, Gonzalez-Espinosa C, Arudchandran R, Xirasagar S, Tybulewicz V, Rivera J: Vav1 regulates phospholipase cgamma activation and calcium responses in mast cells. Mol Cell Biol. 2001 Jun;21 (11) :3763-74., URL: https://www.ncbi.nlm.nih.gov/pubmed/11340169?dopt=, *Ma H, Yankee TM, Hu J, Asai DJ, Harrison ML, Geahlen RL: Visualization of Syk-antigen receptor interactions using green fluorescent protein: differential roles for Syk and Lyn in the regulation of receptor capping and internalization. J Immunol. 2001 Feb 1;166 (3) :1507-16., URL: https://www.ncbi.nlm.nih.gov/pubmed/11160190?dopt=

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The antibody SYK-01 reacts with protein tyrosine kinase p72Syk (Syk; an intracellular antigen), which is required for the transduction of signals through the B cell antigen receptor (BCR) and the high affinity IgE receptor (FcepsilonRI) .

Mouse IgG1