Anti-THEM4 Antilichaam

THEM4, also known as CTMP, binds specifically to the carboxy-terminal regulatory domain of PKB/Akt at the plasma membrane and acts as a negative regulator, reversing the phenotype of v-Akt-transformed cells. Hypermethylation of the THEM4 promoter and transcriptional downregulation of the gene has been reported in multiple glioblastomas, suggesting that epigenetic regulation of THEM4 may play a role in the progression of this cancer. Bioinformatic analysis, confirmed by in vitro testing, indicates that THEM4 is a broad-range, high activity acyl-CoA thioesterase. Recent reports have also indicated that TMEM4 is a mitochondrial protein whose overexpression is associated with an increase in mitochondrial membrane depolarization and caspase-3 and PARP cleavage, suggesting that THEM4 is involved in the apoptotic program.

Boster Bio Anti-THEM4 Antibody (Catalog # A07814) . Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

CTMP, CTMP, Acyl-coenzyme A thioesterase THEM4, Carboxyl-terminal modulator protein, Acyl-CoA thioesterase THEM4

THEM4

Q5T1C6

Human, Mouse, Rat

The additional higher molecular weight bands seen in the immunoblot may represent post-translationally modified TMEM4.

THEM4 antibody was raised against an 18 amino acid synthetic peptide near the center of human THEM4. The immunogen is located within amino acids 60 - 110 of THEM4.

Expressed predominantly in skeletal muscle, testis, uterus, brain and kidney. Down-regulated in glioblastoma or glioma compared to non-neoplastic brain due to promoter hypermethylation.

ELISA, WB, IHC-P, IF

Apoptosis

THEM4 Antibody is affinity chromatography purified via peptide column.

1 mg/mL

Liquid

Has acyl-CoA thioesterase activity towards medium and long-chain (C14 to C18) fatty acyl-CoA substrates, and probably plays an role in mitochondrial fatty acid metabolism. Plays a role in the apoptotic process, possibly via its regulation of AKT1 activity. According to PubMed:11598301, inhibits AKT1 phosphorylation and activity. According to PubMed:17615157, enhances AKT1 activity by favoring its phosphorylation and translocation to plasma membrane.

THEM4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

https://www.bosterbio.com/msds?sku=A07814

27130 MW

Thioesterase superfamily member 4

Cell membrane

Thioesterase superfamily member 4

IgG

THEM4 Antibody is supplied in PBS containing 0.02% sodium azide.