Anti-TOP3B Antibody Picoband®

DNA topoisomerase 3-beta-1 is an enzyme that in humans is encoded by the TOP3B gene. This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus relaxing the supercoils and altering the topology of DNA. The enzyme interacts with DNA helicase SGS1 and plays a role in DNA recombination, cellular aging and maintenance of genome stability. Low expression of this gene may be related to higher survival rates in breast cancer patients. This gene has a pseudogene on chromosome 22. Alternate splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known.

Boster Bio Anti-TOP3B Antibody Picoband® catalog # A10446-2. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

E3 ubiquitin-protein ligase RNF169; RING finger protein 169; RING-type E3 ubiquitin transferase RNF169; RNF169; KIAA1991

TOP3B

8940

O95985

Human, Mouse, Rat

No cross-reactivity with other proteins.

E.coli-derived human TOP3B recombinant protein (Position: E128-R824) .

Expressed in immature but not mature T-cells. Also found in CD34+ cells from peripheral blood, CD34+ precursors from umbilical cord blood and adult bone marrow.

WB, IHC, ELISA

Hematopoietic Progenitors, Lymphoid, Stem Cells, T Lymphocytic Lineage

Immunogen affinity purified.

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Lyophilized

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Probable E3 ubiquitin-protein ligase that acts as a negative regulator of double-strand breaks (DSBs) repair following DNA damage. Recruited to DSB repair sites by recognizing and binding ubiquitin catalyzed by RNF168 and competes with TP53BP1 and BRCA1 for association with RNF168-modified chromatin, thereby acting as a negative regulator of DSBs repair. E3 ubiquitin-protein ligase activity is not required for regulation of DSBs repair.

1. Kaufman, C. S., Genovese, A., Butler, M. G. Deletion of TOP3B is associated with cognitive impairment and facial dysmorphism. Cytogenet. Genome Res. 150: 106-111, 2016. 2. Kawasaki, K., Minoshima, S., Nakato, E., Shibuya, K., Shintani, A., Schmeits, J. L., Wang, J., Shimizu, N. One-megabase sequence analysis of the human immunoglobulin gamma gene locus. Genome Res. 7: 250-261, 1997. 3. Kwan, K. Y., Moens, P. B., Wang, J. C. Infertility and aneuploidy in mice lacking a type IA DNA topoisomerase III-beta. Proc. Nat. Acad. Sci. 100: 2526-2531, 2003.

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

https://www.bosterbio.com/msds?sku=A10446-2

41409 MW

90-97 kDa

Ring finger protein 169

Nucleoplasm.

Protein NDRG3

Rabbit IgG

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.