Anti-TRAM2 Antibody Picoband®

TRAM2 (Translocation-Associating Membrane Protein 2), also called KIAA0057, is a protein that in humans is encoded by the TRAM2 gene. TRAM2 is a component of the translocon, a gated macromolecular channel that controls the posttranslational processing of nascent secretory and membrane proteins at the endoplasmic reticulum (ER) membrane. By genomic sequence analysis, Onuchic et al. (1999) mapped the TRAM2 gene to chromosome 6p21.1-p12 near the PKHD1 gene. Upon activation, quiescent hepatic stellate cells proliferate, change morphologically into myofibroblasts, and increase their synthesis of extracellular matrix proteins. Stefanovic et al. (2004) demonstrated that both TRAM2 and collagen type I are upregulated in activated rat and human hepatic stellate cells.

Boster Bio Anti-TRAM2 Antibody catalog # PA2011. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Translocating chain-associated membrane protein 2; Tram2

TRAM2

170829

Q924Z5

Human, Mouse, Rat

No cross-reactivity with other proteins

A synthetic peptide corresponding to a sequence at the C-terminus of mouse TRAM2, identical to the related rat sequence, and different from the related human sequence by one amino acid.

WB

Organelle Proteins, Protein Trafficking, Signal Transduction

Immunogen affinity purified.

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Lyophilized

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Necessary for collagen type I synthesis. May couple the activity of the ER Ca (2+) pump SERCA2B with the activity of the translocon. This coupling may increase the local Ca (2+) concentration at the site of collagen synthesis, and a high Ca (2+) concentration may be necessary for the function of molecular chaperones involved in collagen folding (By similarity) . .

1. Nomura, N., Nagase, T., Miyajima, N., Sazuka, T., Tanaka, A., Sato, S., Seki, N., Kawarabayasi, Y., Ishikawa, K., Tabata, S. Prediction of the coding sequences of unidentified human genes. II. The coding sequences of 40 new genes (KIAA0041-KIAA0080) deduced by analysis of cDNA clones from human cell line KG-1. DNA Res. 1: 223-229, 1994. 2. Onuchic, L. F., Mrug, M., Lakings, A. L., Muecher, G., Becker, J., Zerres, K., Avner, E. D., Dixit, M., Somlo, S., Germino, G. G., Guay-Woodford, L. M. Genomic organization of the KIAA0057 gene that encodes a TRAM-like protein and its exclusion as a polycystic kidney and hepatic disease 1 (PKHD1) candidate gene. Mammalian Genome 10: 1175-1178, 1999. 3. Stefanovic, B., Stefanovic, L., Schnabl, B., Bataller, R., Brenner, D. A. TRAM2 protein interacts with endoplasmic reticulum Ca (2+) pump Serca2b and is necessary for collagen type I synthesis. Molec. Cell. Biol. 24: 1758-1768, 2004.

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

https://www.bosterbio.com/msds?sku=PA2011

43183 MW

56 kDa

No cross reactivity with other proteins.

Translocating chain-associated membrane protein 2

Membrane ; Multi-pass membrane protein .

Belongs to the TRAM family.

Translocating chain-associated membrane protein 2

Rabbit IgG

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.