Anti-TRPM7 (Ser-1493), Fosfospecifiek antilichaam

The transient receptor potential melastatin (TRPM) subfamily of cation-permeable TRP channels is ubiquitously expressed in mammalian tissues. This family includes TRPM1-8. In addition to acting as a calcium-permeant channel, TRPM6 and TRPM7 possess an inherent serine/threonine kinase activity. TRPM7 specifically is involved with cellular magnesium homeostasis and neurotransmitter release. Due to the magnesium inhibition, TRPM7’s ion channel activity is very low. TRPM7 has been implicated in cell proliferation and migration during cancer progression, and its expression levels correlate with prognosis in breast cancer. TRPM7 kinase activation leads to massive autophosphorylation of the C-terminal region, including phosphorylation of Ser-1493, Ser-1513, and Ser-1569. Both Ser-1513 and Ser-1569 phosphorylation is required for kinase activity, and phosphorylation of Ser-1513 may inhibit Caspase-mediated cleavage of the C-terminal tail. Thus, TRPM7 is a multifunctional transmembrane protein with roles in cell signaling, proliferation, migration, and death.

Our TRPM7 (Ser-1493) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human TRPM7 (Ser-1493) and is antigen affinity purified. It is great for use in ELISA, WB.

Our TRPM7 (Ser-1493) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human TRPM7 (Ser-1493) and is antigen affinity purified. It is great for use in WB.

TRPM7 TrpC7, LTrpC-7, ChaK1, LTRPC7, TRP

TRPM7

Q96QT4

Ser-1493

Human

Phospho-TRPM7 (Ser-1493) synthetic peptide (coupled to carrier) corresponding to amino acids surrounding Ser-1493 in human TRPM7. This site is conserved in rat and mouse TRPM7, but the homology surrounding the site is low. This site has no homology to other TRPM family members.

TRPM7 (Ser-1493)

Unconjugated

ELISA, WB

Ion Channels and Modulators, Trp Channels, Cell Signaling, Kinase Signaling, Oncoproteins

Antigen Affinity Purified

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Phosphorylated

220

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Blue Ice

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Affinity purified rabbit serum

1:500

The antibody was cross-adsorbed to unphosphorylated TRPM7 (Ser-1493) then affinity purified using phospho-TRPM7 (Ser-1493) peptide. This antibody detects a 220 kDa* protein on SDS-PAGE immunoblots of human MDA-MB-231 cells stimulated with calyculin A, and detects a 70 kDa autophosphorylated recombinant kinase domain of human TRPM7. These reactivities are not observed after lambda phosphatase treatment to dephosphorylate TRPM7.

Western blots performed on each lot.

Rabbit

Liquid

IgG

Human

Quality Control: WB

AB_3068519