Anti-β-Tubuline-antilichaam

Microtubules (MTs) are cytoskeletal elements that play an essential role in cell division and cytoplasmic organization. MTs are dynamic polymers of α/β-tubulin heterodimers. At least two populations of MTs, called dynamic and stable according to their rates of turnover, are readily distinguishable in cells. The proteins associated with MTs (MAPs) are among the best-known factors that regulate MT dynamics and stability. In addition, a variety of different post-translational modifications may also regulate MT dynamics and stability. Phosphorylation is one of these modifications and it can occur on serine, threonine, and tyrosine residues in β-Tubulin isoforms. Multiple kinases can phosphorylate Ser-444 at the C-terminus of βIII-Tubulin in vitro. Unphosphorylated Ser-444 in βIII-Tubulin is an early marker for cells of neuronal lineage, while phosphorylation of Ser-444 is upregulated after neuronal maturation and may preferentially occur in assembled MTs. By contrast, Cdk1 phosphorylation of Ser-172 in β-Tubulin occurs in mitotic cells and may impair tubulin incorporation into microtubules.

Our β-Tubulin primary antibody from PhosphoSolutions is mouse monoclonal. It detects human, mouse, and rat β-Tubulin and is protein a purified. It is great for use in ELISA, WB, ICC.

Our β-Tubulin primary antibody from PhosphoSolutions is mouse monoclonal. It detects human, mouse, and rat β-Tubulin and is Protein A purified. It is great for use in WB, ICC.

TUBB3

TUBB3

P02554

Human, Mouse, and Rat

Clone (M154) was generated from purified porcine brain tubulin.

β-Tubulin

M154

Unconjugated

ELISA, WB, ICC

Cell Adhesion Molecules, Cell Markers, Cytoskeletal Markers, Cell Signaling, Cytoskeletal Regulation

Protein A Purified

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

50

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Blue Ice

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Protein A purified culture supernatant.

1:1000

This antibody detects a 50 kDa* protein corresponding to the molecular mass of β-Tubulin on SDS-PAGE immunoblots of purified brain tubulin, mouse brain tissue, rat PC12 cells, and human A431 and SH-SY5Y cells.

1:200

Western blots performed on each lot.

Mouse

Liquid

IgG1

Pig

Quality Control: WB

AB_760244