Anti-Ubc13/UBE2N Antibody Picoband® fluoro647 Vervoeging

Ubiquitin-conjugating enzyme E2 N is a protein that in humans is encoded by the UBE2N gene. The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Studies in mouse suggest that this protein plays a role in DNA postreplication repair.

Probable ATP-dependent RNA helicase DDX4; DEAD box protein 4; Vasa homolog; DDX4; VASA

UBE2N

7334

P61088

Human, Mouse, Rat

No cross-reactivity with other proteins.

E.coli-derived human Ubc13/UBE2N recombinant protein (Position: Q13-I152) .

Expressed only in ovary and testis. Expressed in migratory primordial germ cells in the region of the gonadal ridge in both sexes.

Flow Cytometry

Signal Transduction, Cell Biology, Cell Cycle, Stem Cells, Cell Differentiation, Cytoskeleton/ECM, Extracellular Matrix, Structures, ECM Proteins, Mesenchymal Stem Cells

Immunogen affinity purified.

Liquid

ATP-dependent RNA helicase required during spermatogenesis (PubMed:10920202, PubMed:21034600) . Required to repress transposable elements and preventing their mobilization, which is essential for the germline integrity (By similarity) . Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons (By similarity) . Involved in the secondary piRNAs metabolic process, the production of piRNAs in fetal male germ cells through a ping-pong amplification cycle (By similarity) . Required for PIWIL2 slicing-triggered piRNA biogenesis: helicase activity enables utilization of one of the slice cleavage fragments generated by PIWIL2 and processing these pre-piRNAs into piRNAs (By similarity) .

1. Chen, J., Hao, L., Li, C., Ye, B., Du, Y., Zhang, H., Long, B., Zhu, P., Liu, B., Yang, L., Li, P., Tian, Y., Fan, Z. The endoplasmic reticulum adaptor protein ERAdP initiates NK cell activation via the Ubc13-mediated NF-kappa-B pathway. J. Immun. 194: 1292-303, 2015. 2. Fukushima, T., Matsuzawa, S., Kress, C. L., Bruey, J. M., Krajewska, M., Lefebvre, S., Zapata, J. M., Ronai, Z., Reed, J. C. Ubiquitin-conjugating enzyme Ubc13 is a critical component of TNF receptor-associated factor (TRAF) -mediated inflammatory responses. Proc. Nat. Acad. Sci. 104: 6371-6376, 2007. 3. Gross, M. B. Personal Communication. Baltimore, Md. 7/25/2014.

At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

38779 MW

6

DEAD-box helicase 4

Secreted, extracellular space, extracellular matrix.

BAG family molecular chaperone regulator 1

Rabbit IgG

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.