Anti-Vav2 Antilichaam

The Vav family of Rho-guanine nucleotide exchange factors, Vav1, Vav2, and Vav3, have central roles in transducing signals from cell surface receptors, such as integrin, growth factor and immune cell receptors to the cytoskeleton. This role includes receptor-mediated changes in the actin cytoskeleton and cell motility. Vav1 expression is normally restricted to hematopoietic cells, while Vav2 and Vav3 are more widely expressed. All three Vav isoforms have been shown to be abnormally expressed in several types of cancer. Vavs are composed of multiple domains, including a Dbl homology domain, a calponin homology domain, an acidic amino acid region, a pleckstrin homology domain, a cysteine-rich domain, and SH3 and SH2 domains. Vav activity is regulated by the phosphorylation status of several conserved tyrosine residues in the acidic region (In Vav2: Tyr-142, Tyr-159, and Tyr-172) . These tyrosine residues are able to participate in autoinhibitory interactions with the Dbl homology domain and this interaction is prevented after phosphorylation of these sites leading to activation of Vav GEF activity.

Our Vav2 rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat Vav2 and is antigen affinity purified. It is great for use in ELISA, WB, ICC, IP.

Our Vav2 rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat Vav2 and is antigen affinity purified. It is great for use in WB, ICC, IP.

VAV2, Guanine nucleotide exchange factor VAV2

VAV2

Q60992

Human, Mouse, and Rat

Vav2 synthetic peptide (coupled to KLH) corresponding to amino acid residues surrounding Tyr-172 in mouse Vav2. This sequence has high homology with similar regions in rat and human Vav2, and has significant homology to the conserved site in Vav1 and Vav3.

Vav2

Unconjugated

ELISA, WB, ICC, IP

Cell Signaling, Cytoskeletal Regulation, G Protein Signaling, Growth Factor Signaling, Oncoproteins

Antigen Affinity Purified

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

100

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Blue Ice

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Affinity purified rabbit serum

1:1000

1:100

The antibody detects a 100 kDa* band corresponding to Vav2 on SDS-PAGE immunoblots of human A431 cells and detects several Vav isoforms in Jurkat cells.

1:50

Western blots performed on each lot.

Rabbit

Liquid

IgG

Mouse

Quality Control: WB

AB_1184726