Anti-Vimentin-antilichaam

Intermediate filaments (IF) are important components of the cytoskeleton and nuclear envelope. IF protein family members are encoded by 70 genes that have diverse expression patterns during development and tissue-specific functions. For example, the keratins form IFs in epithelial cells, while vimentin forms filaments in mesenchymal, endothelial, and hematopoietic cells. Neurofilament triplet proteins and internexins form IFs in neurons, while desmin, synemin and syncoilin form the extra-sarcomeric cytoskeleton of myoblasts. The nuclear IF system consists of lamin family proteins organized in a meshwork-like lamina around the nucleus. Vimentin is a 58 kDa IF protein that forms 10 nm filaments that are important for mechanical stability, motility, and adhesion in various cell types. Vimentin activity is regulated by proteolysis and phosphorylation. Rho-kinases can phosphorylate vimentin leading to destabilization of intermediate filaments, while Akt phosphorylation of Ser-39 in vimentin reduces proteolysis of vimentin and promotes cancer cell motility and invasion.

Our Vimentin primary antibody from PhosphoSolutions is mouse monoclonal. It detects chicken, human, mouse, and rat Vimentin and is protein a purified. It is great for use in ELISA, WB, ICC.

Our Vimentin primary antibody from PhosphoSolutions is mouse monoclonal. It detects chicken, human, mouse, and rat Vimentin and is Protein A purified. It is great for use in WB, ICC.

VIM

VIM

P02543

Chicken, Human, Mouse, and Rat

Clone (V9) was generated from full length vimentin purified from porcine eye lens. The antibody reacts with human, pig, chicken, rat, and mouse vimentins.

Vimentin

V9

Unconjugated

ELISA, WB, ICC

Apoptosis, Cell Markers, Cytoskeletal Markers, Oncoproteins

Protein A Purified

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

58

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Blue Ice

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Protein A purified culture supernatant.

1:200

The antibody detects a 58 kDa* protein corresponding to the molecular mass of vimentin on SDS-PAGE immunoblots of human fibroblasts (HS-68) . In immunocytochemistry, the antibody detects intermediate filaments in rat smooth muscle cells (A7r5) .

1:100

Western blots performed on each lot.

Mouse

Liquid

IgG1

Pig

Quality Control: WB

AB_3068524