Anti-WAVE1 (N-terminaal gebied) Antilichaam

The Wiskott–Aldrich syndrome protein (WASP) family is involved in various pathways that regulate actin cytoskeletal organization. This family includes WASP, N-WASP, and three WAVE/SCAR isoforms, WAVE1, 2, and 3. WAVE proteins play key roles in actin-mediated cell events, such as membrane ruffling and lamellipodia formation. WAVEs contain an N-terminal WAVE homology domain, a basic domain, a Proline-rich region, and carboxy terminal verprolin, cofilin, and acidic (VCA) region. WAVEs are thought to act downstream of the Rac GTPase, connecting Rac activation to induction of Arp 2/3-mediated actin polymerization. Regulation of WAVE activity can occur through tyrosine phosphorylation. Src phosphorylation of WAVE1 at Tyr-125 enhances binding to the Arp2/3 complex, and is required for WAVE inhibition of Arp2/3-mediated stress fiber formation. By contrast, WAVE2 phosphorylation of Tyr-150 by Abl may enhance Arp2/3 complex actin nucleation and microspike formation in fibroblasts. Thus, site-specific tyrosine phosphorylation may be important for controlling specific activities of WAVE proteins.

Our WAVE1 (N-terminal region) rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat WAVE1 (N-terminal region) and is antigen affinity purified. It is great for use in ELISA, WB, ICC.

Our WAVE1 (N-terminal region) rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat WAVE1 (N-terminal region) and is antigen affinity purified. It is great for use in WB, ICC.

Wiskott-Aldrich syndrome verproline, Scar1, WASF1

WASF1

Q92558

Human, Mouse, and Rat

A synthetic peptide (coupled to KLH) corresponding to amino acid residues in the N-terminal half of human WAVE1. This sequence has high homology with similar regions in rat and mouse WAVE1, and has less than 50% homology to similar regions in human WAVE2 and WAVE3.

WAVE1 (N-terminal region)

Unconjugated

ELISA, WB, ICC

Cell Signaling, Cytoskeletal Regulation

Antigen Affinity Purified

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

80

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Blue Ice

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Affinity purified rabbit serum

1:1000

The antibody detects an 80 kDa* protein corresponding to the molecular mass of WAVE1 on SDS-PAGE immunoblots of human SYF cSrc-transformed, HUVEC, and K562 cells, as well as rat PC12 cells and mouse C2C12 cells.

1:200

Western blots performed on each lot.

Rabbit

Liquid

IgG

Human

Quality Control: WB

AB_2241749