Anti-WWP1 (N-terminus) Antilichaam

The Nedd4-like family of E3 ubiquitin ligases have been implicated in several types of human cancer. There are nine members of the Nedd4-like E3 family, all of which have an N-terminal C2 domain, two to four WW domains in the central region, and a C-terminal domain that is homologous to the C-terminus of E6-AP. This ubiquitin ligase family includes NEDD4-1, NEDD4-2, AIP4/Itch, AIP5/WWP1, SMURF1 and SMURF2. Several ubiquitin ligases in this family are overexpressed in human cancers. WWP1 has been implicated as an oncogene as it is overexpressed in prostate and breast cancers, and knockdown of this ligase reduces cell proliferation and causes apoptosis. In addition, WWP1 has been shown to negatively regulate transforming growth factor-β (TGFβ) signaling by targeting TGFβ receptor 1, Smad2, and Smad4 for ubiquitin-mediated degradation. WWP1 has also been shown to regulate the protein levels of the epithelial sodium channel (ENaC), Notch, EGFR/ErbB2, Runx2, KLF2, KLF5, p53, and p63. Thus, WWP1 may regulate cell development and proliferation through the degradation of proteins involved in a variety of cell signaling pathways.

Our WWP1 (N-terminus) rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects chicken, human, mouse, and rat WWP1 (N-terminus) and is antigen affinity purified. It is great for use in ELISA, WB.

Our WWP1 (N-terminus) rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects chicken, human, mouse, and rat WWP1 (N-terminus) and is antigen affinity purified. It is great for use in WB.

AIP5, Tiul1; hSDRP1

WWP1

Q9H0M0

Chicken, Human, Mouse, and Rat

WWP1 synthetic peptide (coupled to carrier protein) corresponds to amino acids in the N-terminus of human WWP1. This sequence is highly conserved in rat and mouse WWP1, and has low homology to other NEDD4-like family members.

WWP1 (N-terminus)

Unconjugated

ELISA, WB

Cell Signaling, Oncoproteins

Antigen Affinity Purified

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

110

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Blue Ice

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Affinity purified rabbit serum

1:1000

The antibody detects a 110 kDa* protein corresponding to the apparent molecular mass of WWP1 on SDS-PAGE immunoblots of adult mouse liver and human PC3 cells. The antibody also detects lower molecular weight isoforms of WWP1 in mouse liver, and human PC3, HepG2, K562, HeLa, and HUVEC.

Western blots performed on each lot.

Rabbit

Liquid

IgG

Human

Quality Control: WB

AB_10552714