Antichromogranin A/Chga Antibody Picoband® fluoro550 Vervoeging

CHGA, also known as CGA or chromogranin A, is an acidic protein costored and coreleased with catecholamines from storage granules in the adrenal medulla. CHGA is a member of the granin family of neuroendocrine secretory protein, It is located in secretory vesicles of neurons and endocrine cells. In humans, CHGA protein is encoded by the CHGA gene, and it is mapped to 14q32.12. CHGA gene product is a precursor to three biologically active peptides: vasostatin, pancreastatin, and parastatin. These peptides negatively modulate the neuroendocrine function of the releasing cell (autocrine) or nearby cells (paracrine) .

Kit ligand; Mast cell growth factor; MGF; Stem cell factor; SCF; c-Kit ligand; Soluble KIT ligand; sKITLG; KITLG; MGF, SCF

Chga

24258

P10354

Mouse, Rat

No cross-reactivity with other proteins.

A synthetic peptide corresponding to a sequence at the N-terminus of rat Chromogranin A/Chga, which shares 77.8% and 100% amino acid (aa) sequence identity with human and mouse CHGA, respectively.

Flow Cytometry

Cytokines, Developmental Biology, Embryogenesis, Embryonic Stem Cells, Immunology, Innate Immunity, Stem Cells, Surface Molecules

Immunogen affinity purified.

Liquid

Ligand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG/SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5- trisphosphate. KITLG/SCF acts synergistically with other cytokines, probably interleukins.

1. Helman LJ, Ahn TG, Levine MA, Allison A, Cohen PS, Cooper MJ, Cohn DV, Israel MA (August 1988) . Molecular cloning and primary structure of human chromogranin A (secretory protein I) cDNA. J. Biol. Chem. 263 (23) : 11559–63. 2. Cotesta D, Caliumi C, Alò P, Petramala L, Reale MG, Masciangelo R, Signore A, Cianci R, D'Erasmo E, Letizia C (2005) . High plasma levels of human chromogranin A and adrenomedullin in patients with pheochromocytoma. Tumori 91 (1) : 53–8.

At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

6

KIT ligand

Isoform 1: Cell membrane; Single-pass type I membrane protein.

Rabbit IgG

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.