Anticofiline 1 (N-terminus) Antilichaam

Members of the ADF/cofilin (AC) family are actin-severing proteins that regulate actin remodeling during cellular events such as cell migration, cytokinesis, phagocytosis, endocytosis, axon development, and immune cell activation. In mammals, there are three members of the AC family, muscle-specific cofilin (cofilin 2), non-muscle cofilin (cofilin 1), and ADF. In humans, cofilin 1 and ADF have 72% identity, with the major amino acid differences found in the C-terminal region. Regulation of cofilin activity can occur through serine phosphorylation. Activation of cofilin kinases, LIMK1 or LIMK2, leads to phosphorylation of cofilin at serine 3. This phosphorylation disrupts cofilin binding to actin in vitro and in vivo. Multiple phosphatases, PP1, PP2A, PP2B, slingshot, and chronophin can dephosphorylate Ser-3 and activate actin binding. Thus, Ser-3 phosphorylation is a major site for the regulation of cofilin activity.

Our Cofilin 1 (N-terminus) rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects chicken, human, mouse, and rat Cofilin 1 (N-terminus) and is antigen affinity purified. It is great for use in ELISA, WB, ICC.

Our Cofilin 1 (N-terminus) rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects chicken, human, mouse, and rat Cofilin 1 (N-terminus) and is antigen affinity purified. It is great for use in WB, ICC.

ADF, p18

CFL1

P23528

Chicken, Human, Mouse, and Rat

A synthetic peptide (coupled to KLH) corresponding to amino acid residues at the N-terminus of human Cofilin 1. This sequence has 100% homology with similar regions of rat and mouse Cofilin 1, and has 3 amino acid differences from human Cofilin 2.

Cofilin 1 (N-terminus)

Unconjugated

ELISA, WB, ICC

Cell Signaling, Cytoskeletal Regulation, Oncoproteins

Antigen Affinity Purified

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

19

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Blue Ice

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Affinity purified rabbit serum

1:1000

The antibody detects a 19 kDa* protein corresponding to the molecular mass of Cofilin 1 on SDS-PAGE immunoblots of Jurkat cells.

1:50

Western blots performed on each lot.

Rabbit

Liquid

IgG

Human

Quality Control: WB

AB_2260620