Anticofiline/CFL1 Antibody Picoband® fluoro488 Vervoeging

Cofilin, a member of the ADF/cofilin family, is actually a protein with 70% sequence homology to ADF, making it part of the ADF/cofilin family of small ADP-binding proteins. It is a ubiquitous actin-binding factor required for the reorganization of actin filaments. The protein is known to sever actin filaments by creating more positive ends on filament fragments, it can cause depolymerization at the minus end of filaments, thereby preventing their reassembly. As a long-lasting in vivo effect, cofilin recycles older ADP-F-actin, helping cell to maintain ATP-G-actin pool for sustained motility. pH, phosphorylation and phosphoinositides regulate cofilin’s binding and associating activity with actin.

Cofilin-1;18 kDa phosphoprotein; p18; Cofilin, non-muscle isoform; CFL1; CFL

CFL1

1072

P23528

Human, Monkey, Mouse, Rat

No cross-reactivity with other proteins

E.coli-derived human Cofilin recombinant protein (Position: A2-L166) . Human Cofilin shares 99% amino acid (aa) sequences identity with both mouse and rat Cofilin.

Widely distributed in various tissues.

Flow Cytometry

Actin Binding Proteins, Actin, etc., Axonal Guidance Proteins, Cancer, Cardiovascular, Cell Adhesion, Cell Type Marker, Contractility, Cytoskeleton, Cytoskeleton/ECM, Growth and Development, Invasion/Microenvironment, Microfilaments, Neurology Process, Neuron Marker, Neuroscience, Signal Transduction, Vasculature

Immunogen affinity purified.

Liquid

Binds to F-actin and exhibits pH-sensitive F-actin depolymerizing activity. Regulates actin cytoskeleton dynamics. Important for normal progress through mitosis and normal cytokinesis. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required for the up-regulation of atypical chemokine receptor ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation. .

1. Cooper, G. M. and R. E. Hausman. The Cell: A Molecular Approach, 3rd ed. Washington DC: ASM Press 2004 pp.436-440. 2. Ichetovkin I, Han J, Pang KM, Knecht DA, Condeelis JS (April 2000) . Actin filaments are severed by both native and recombinant dictyostelium cofilin but to different extents. Cell Motil. Cytoskeleton 45 (4) : 293–306. 3. Lappalainen P, Drubin DG (July 1997) . Cofilin promotes rapid actin filament turnover in vivo.Nature 388 (6637) : 78–82.

At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

18502 MW

No cross reactivity with other proteins.

6

Cofilin-1

Nucleus matrix . Cytoplasm, cytoskeleton . Cell projection, ruffle membrane ; Peripheral membrane protein ; Cytoplasmic side . Cell projection, lamellipodium membrane ; Peripheral membrane protein ; Cytoplasmic side . Colocalizes with the actin cytoskeleton in membrane ruffles and lamellipodia. Detected at the cleavage furrow and contractile ring during cytokinesis. Almost completely in nucleus in cells exposed to heat shock or 10% dimethyl sulfoxide.

Belongs to the actin-binding proteins ADF family.

Cofilin-1

Rabbit IgG

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.