Antifascine (kloon 55K2) Antilichaam

Fascin is an actin filament bundling protein localized to lamellipodia and filopodia where it has important roles in cell motility. Regulation of fascin occurs through PKC-mediated phosphorylation of Ser-39 in the F-actin binding site. Cell permeant peptides that block PKC phosphorylation of Ser-39 increase cell migration, while peptides that block fascin binding to F-actin alter lamellipodial morphology and cause aberrant cell motility. Studies using RNA interference of fascin show that fibroblasts have reduced number and abnormal morphology of filopodia, while Ser-39 phosphorylation status may determine filopodial frequency. In Drosophila neurons, fascin deficiency causes alterations in actin filaments and leads to abnormal morphology of developing neurons. Thus, fascin is a critical element of actin-based motility in various cell types.

Our Fascin (clone 55K2) primary antibody from PhosphoSolutions is mouse monoclonal. It detects human, mouse, and rat Fascin (clone 55K2) and is protein a purified. It is great for use in ELISA, WB, ICC, IHC.

Our Fascin (clone 55K2) primary antibody from PhosphoSolutions is mouse monoclonal. It detects human, mouse, and rat Fascin (clone 55K2) and is Protein A purified. It is great for use in WB, ICC, IHC.

P55

FSCN1

Q16658

Human, Mouse, and Rat

Clone (55K2) was generated from full-length human fascin purified from HeLa cells. This sequence has high homology to rat and mouse fascin.

Fascin (clone 55K2)

55K2

Unconjugated

ELISA, WB, ICC, IHC

Cell Signaling, Cytoskeletal Regulation

Protein A Purified

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

55

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Blue Ice

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Protein A purified culture supernatant.

1:100

1:1000

1:100

The antibody detects a 55 kDa* band corresponding to fascin on SDS-PAGE immunoblots of human A431 and HeLa, as well as mouse C2C12 and rat PC12.

1:100

Western blots performed on each lot.

Mouse

Liquid

IgG1

Human

Quality Control: WB

AB_2106385