Antiparkinsonziekte eiwit 2 (Parkin) Antilichaam

FUNCTION: Functions within a multiprotein E3 ubiquitin ligase complex, catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins. These substrates include SYT11, CCNE1, GPR37, STUB1, a 22 kDa O-linked glycosylated isoform of SNCAIP and SEPT5. May play a more general role in the ubiquitin proteasomal pathway by participating in the removal and/or detoxification of abnormally folded or damaged protein. Loss of this ubiquitin ligase activity appears to be the mechanism underlying pathogenesis of PARK2. May protect neurons against alpha synuclein toxicity, proteasomal dysfunction, GPR37 accumulation, and kainate-induced excitotoxicity. May play a role in controlling neurotransmitter trafficking at the presynaptic terminal and in calcium-dependent exocytosis. Regulates cyclin E during neuronal apoptosis. May represent a tumor suppressor gene. SUBCELLULAR LOCATION: Cytoplasm. Co-localizes with STY11 in neutrites. Co-localizes with SNCAIP in brainstem Lewy bodies. TISSUE SPECIFICITY: Highly expressed in the brain including the substantia nigra. Expressed in heart, testis and skeletal muscle. Expression is down-regulated or absent in tumor biopsies, and absent in the brain of PARK2 patients. Overexpression protects dopamine neurons from kainate-mediated apoptosis.

Our Anti-Parkinson disease protein 2 (Parkin) rabbit polyclonal primary antibody detects guinea pig, human, and rat Parkinson disease protein 2 (Parkin), and is whole serum. It is validated for use in ELISA, IHC-Frozen, WB.

Our Anti-Parkinson disease protein 2 (Parkin) rabbit polyclonal primary antibody detects guinea pig, human, and rat Parkinson disease protein 2 (Parkin), and is whole serum. It is validated for use in ELISA, IHC-Frozen, WB.

Ubiquitin E3 ligase PRKN; Parkinson juvenile disease protein 2; Parkinson disease protein 2; PARK2; PRKN

O60260

Guinea Pig, Human, and Rat

A synthetic peptide (RILGEEQYNRYQQYGAEEC) as part of human Parkin conjugated to diphtheria toxoid has been used as the immunogen.

Parkinson disease protein 2 (Parkin)

Unconjugated

ELISA, IHC-Frozen, WB

Whole serum

50 kDa

For research use only.

25°C (ambient)

Spin vial briefly before opening. Reconstitute in 100 μL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material. Store lyophilized antibody at 2-8°C. After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for an additional stability. Avoid repetitive freeze/thaw cycles.

1:500-1:2000

1:500-1:2000

This antiserum is known to specifically recognize Parkin shown by IHC and WB. This antibody is known to react with Parkin of guinea pig and rat.

1:500-1:2000

Rabbit

Lyophilized

Mixed

Human