Antisplicerende factor 1 Antibody Picoband® (monoklonale, 7D9E3)

Splicing factor 1 also known as zinc finger protein 162 (ZFM162) is a protein that in humans is encoded by the SF1 gene. This gene encodes a nuclear pre-mRNA splicing factor. The encoded protein specifically recognizes the intron branch point sequence at the 3' splice site, together with the large subunit of U2 auxiliary factor (U2AF), and is required for the early stages of spliceosome assembly. It also plays a role in nuclear pre-mRNA retention and transcriptional repression. The encoded protein contains an N-terminal U2AF ligand motif, a central hnRNP K homology motif and quaking 2 region which bind a key branch-site adenosine within the branch point sequence, a zinc knuckles domain, and a C-terminal proline-rich domain. Alternative splicing results in multiple transcript variants.

ATP-dependent Clp protease ATP-binding subunit clpX-like, mitochondrial; CLPX

SF1

7536

Q15637

Human, Mouse, Rat

No cross-reactivity with other proteins.

E. coli-derived human splicing factor 1 recombinant protein (Position: R160-Q266) .

Clone: 7D9E3

Higher expression in skeletal muscle and heart and to a lesser extent in liver, brain, placenta, lung, kidney and pancreas.

Flow Cytometry

Adapters, Cytokines, Immunology, Innate Immunity, Interleukins, Macrophage/Inflammation, Signal Transduction, Transmembrane

Immunogen affinity purified.

Liquid

ATP-dependent specificity component of the Clp protease complex. Hydrolyzes ATP (PubMed:28874591) . Targets specific substrates for degradation by the Clp complex (PubMed:11923310, PubMed:22710082) . Can perform chaperone functions in the absence of CLPP. Enhances the DNA-binding activity of TFAM and is required for maintaining a normal mitochondrial nucleoid structure (PubMed:22841477) . ATP-dependent unfoldase that stimulates the incorporation of the pyridoxal phosphate cofactor into 5-aminolevulinate synthase, thereby activating 5-aminolevulinate (ALA) synthesis, the first step in heme biosynthesis (PubMed:28874591) . Important for efficient erythropoiesis through upregulation of heme biosynthesis (PubMed:25957689, PubMed:28874591) .

1. Toda, T., Iida, A., Miwa, T., Nakamura, Y., Imai, T. Isolation and characterization of a novel gene encoding nuclear protein at a locus (D11S636) tightly linked to multiple endocrine neoplasia type 1 (MEN1) . Hum. Molec. Genet. 3: 465-470, 1994. 2. Caslini, C., Spinelli, O., Cazzaniga, G., Golay, J., De Gioia, L., Pedretti, A., Breviario, F., Amaru, R., Barbui, T., Biondi, A., Introna, M., Rambaldi, A. Identification of two novel isoforms of the ZNF162 gene: a growing family of signal transduction and activator of RNA proteins. Genomics 42: 268-277, 1997.

At -20 ̊C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

6

Caseinolytic mitochondrial matrix peptidase chaperone subunit

Mitochondrion; mitochondrion nucleoid

Mouse IgG2b

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.