APG7 Antilichaam

APG7 Antibody: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1. Another member of the autophagy family of proteins is APG7 which was identified in yeast as a ubiquitin-E1-like enzyme; this function is conserved in the mammalian homolog. In mammalian cells, APG7 is essential for autophagy conjugation systems, autophagosome formation, starvation-induced bulk degradation of proteins and organelles. It has been suggested that caspase-8 may alter APG7 levels and thus the APG7 program of autophagic cell death.

10533

O95352

NP_006386

Human, Mouse

Unconjugated

Primary Antibodies

Apoptosis, Autophagy

APG7 Antibody is affinity chromatography purified via peptide column.

Cat. No. 1223 - Caco-2 Cell Lysate

1 mg/mL

Predicted species reactivity based on immunogen sequence: Chicken: (100%), Rat: (100%)

APG7 Antibody is supplied in PBS containing 0.02% sodium azide.

None

Blue Ice

APG7 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

IgG

At least three isoforms of APG7 are known to exist; this antibody will detect all three isoforms. APG7 antibody is predicted not to cross-react with other ATG family proteins.

ATG7

Cat. No. 1219 - MCF7 Cell Lysate

Cat. No. 17-012 - MCF7 Cell Slide

ATG7 autophagy related 7 homolog (S. cerevisiae)

Homo sapiens

Gozuacik D and Kimchi A. Autophagy as a cell death and tumor suppressor mechanism. Oncogene. 2004; 23:2891-906.

Kisen GO, Tessitore L, Costelli P, et al. Reduced autophagic activity in primary rat hepatocellular carcinoma and ascites hepatoma cells. Carcinogenesis 1993; 14:2501-5.

Kamada Y, Funakoshi T, Shintani T, et al. Tor-mediated induction of autophagy via Apg1 protein kinase complex. J. Cell. Biol. 2000; 150:1507-13.

Mizushima N, Noda T, Yoshimori T, et al. A protein conjugation system essential for autophagy. Nature 1998; 395:395-8.

APG7 Antibody: GSA7, APG7L, APG7-LIKE, Ubiquitin-like modifier-activating enzyme ATG7, ATG12-activating enzyme E1 ATG7, APG7-like

ELISA, WB, ICC, IF

5453668

Liquid