APOA1 Antilichaam / Apolipoproteïne A

ELISA: 1-5 µg/mL (Capture), Western Blot: 0.25-0.5 µg/mL

P02647

Human

E. coli-derived mouse Apolipoprotein A I recombinant protein (Position: D25-Q264) was used as the immunogen for the APOA1 antibody.

17G5

ELISA (Cap), WB

Affinity-chromatography

Lyophilized

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

This APOA1 antibody is available for research use only.

After reconstitution, the APOA1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

Optimal dilution of the APOA1 antibody should be determined by the researcher.

Western blot analysis of APOA1 using anti-APOA1 antibody. Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. Lane 1: human HepG2 whole cell lysates, Lane 2: human CACO-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-APOA1 antibody at 0.5 ug/ml overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substratewith Tanon 5200 system. Western blot probed with anti-APOA1 shows a major band at ~25 kDa, lower than the predicted ~31 kDa, consistent with the known anomalous migration of the amphipathic APOA1 protein on SDS-PAGE.