APOBEC3C Antilichaam

Western Blot: 0.25-0.5 µg/mL, Immunohistochemistry: 2-5 µg/mL, Immunocytochemistry: 5 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells

Q9NRW3

Human, Mouse, Rat

A synthetic peptide corresponding to a sequence at the N-terminus of human APOBEC3C was used as the immunogen for the APOBEC3C antibody.

WB, IHC, ICC, IF, FACS

Immunogen affinity purified

Lyophilized

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

This APOBEC3C antibody is available for research use only.

After reconstitution, the APOBEC3C antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

Optimal dilution of the APOBEC3C antibody should be determined by the researcher.

Nuclear, cytoplasmic

Immunofluorescent staining of APOBEC3C using anti-APOBEC3C antibody (green) and anti-Beta Tubulin antibody (red) . APOBEC3C was detected in immunocytochemical section of HELA cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/ml rabbit anti-APOBEC3C antibody and mouse anti-Beta Tubulin antibody overnight at 4oC. DyLight 488 Conjugated Goat Anti-Rabbit IgG and Cy3 Conjugated Goat Anti-Mouse IgG were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37oC. Visualize using a fluorescence microscope and filter sets appropriate for the label used.