APRIL Antilichaam [Saccha-2]

8741;9370

O75888 ; Q15848

Human, Mouse

APRIL (monoclonal Sacha-2) antibody was raised against recombinant human APRIL (aa. 105-250) .

Sacha-2

Unconjugated

Primary Antibodies

Chemokines & Cytokines, Cancer

APRIL (monoclonal Sacha-2) antibody was purified from concentrated hybridoma tissue culture supernatant. APRIL (monoclonal Sacha-2) antibody was purified to 95% in SDS-PAGE.

Batch dependent

PBS containing 0.02% sodium azide

None

Blue Ice

APRIL antibody can be stored at 4˚ C, stable for one year.

IgG2a

APRIL antibody can be used to recognize human and mouse APRIL by ELISA (capture), Flow Cytometry, and Immunocytochemistry. Method: HEK-293T cells expressing surface membrane associated human APRIL (aa 92-233) or a mock control were injected in chicken liver and frozen in OCT. 8 μ m sections of injected frozen liver were fixed in acetone, washed in PBS and incubated with Sacha-2 at 10 μ g/mL for 1hr after blocking in PBS/1%BSA. Human APRIL was revealed with 3-amino-9-Ethyl-carbazole (AEC) following 45 min. incubation with goat anti-rat HRP. Method: HEK 293T cells (5 x 105) were mock transfected (thin line) or transfected with an expression plasmid enabling surface expression of mouse APRIL (thick line) . Cells were incubated on ice for 30 min in 50 μ l FACS buffer (PBS, 5% Fetal calf serum, 0.02% sodium azide) containing 1 μ g/mL of Sacha-2 antibody. After washing in FACS buffer, PE-conjugated antibody to rat IgG was added. Cells were incubated on ice for 30 min, washed and then analyzed by flow cytometry. This product is for research use only.

Tumor necrosis factor (ligand) superfamily, member 13 / adiponectin, C1Q and collagen domain containing

Homo sapiens

ELISA, ICC, Flow

Liquid