Aquaporine 4 Antilichaam / AQP4

In skeletal muscle, AQP4 (aquaporin 4 also known as mercurial insensitive water channel), localizes to the sarcolemma of fast-twitch muscle fibers. Aquaporins (AQPs) are a large family of integral membrane water transport channel proteins that facilitate the transport of water through the cell membrane. This function is conserved in animals, plants and bacteria. Many isoforms of aquaporin have been identified in mammals, designated AQP0through AQP10. Aquaporins are widely distributed and it is not uncommon for more than one type of AQP to be present in the same cell. Although most aquaporins are only permeable to water, AQP3, AQP7, AQP9 and one of the two AQP10 transcripts are also permeable to urea and glycerol. AQP2 is the only water channel that is activated by vasopressin to enhance water reabsorption in the kidney collecting duct. Aquaporins are involved in renal water absorption, generation of pulmonary secretions, lacrimation and the secretion and reabsorption of cerebrospinal fluid and aqueous humor.

Immunohistochemistry (FFPE) : 1-2 µg/mL, Western blot: 2-4 µg/mL

P55087

Human

A human partial recombinant protein (from within amino acids 200-323) was used as the immunogen for the Aquaporin 4 antibody.

AQP4/3321

IHC-P, WB

Protein G affinity

Purified

This Aquaporin 4 antibody is available for research use only.

Aliquot the Aquaporin 4 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Optimal dilution of the Aquaporin 4 antibody should be determined by the researcher.

Cell membrane, cytoplasm

Western blot testing of human brain tissue lysate with Aquaporin 4 antibody (clone AQP4/3321) . Observed molecular weight: 35~45kDa depending on glycosylation level.