ARAF Antilichaam / A-Raf

Serine/threonine-protein kinase A-Raf or simply A-Raf is an enzyme that in humans is encoded by the ARAF gene. It is mapped to Xp11.3. This proto-oncogene belongs to the RAF subfamily of the Ser/Thr protein kinase family, and maybe involved in cell growth and development. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. A-Raf is a member of the Raf kinase family of serine/threonine-specific protein kinases.

Western blot: 0.5-1 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL

P10398

Human, Mouse, Rat

Amino acids E309-T413 from the human protein were used as the immunogen for the ARAF antibody.

WB, IF, FACS, Direct ELISA

Affinity purified

Antigen affinity purified

Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

This ARAF antibody is available for research use only.

Store the ARAF antibody at -20°C.

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Optimal dilution of the ARAF antibody should be determined by the researcher.

Cytoplasmic

Immunofluorescent staining of FFPE human MCF7 cells with ARAF antibody (green) and DAPI nuclear stain (blue) . HIER: steam section in pH6 citrate buffer for 20 min.