Arginase 1 Antilichaam / ARG1

Recognizes a protein of 35-38kDa, which is identified as Arginase 1 (ARG1) . Arginase is a manganese metallo-enzyme that catalyzes the hydrolysis of arginine to generate ornithine and urea. Arginase I and II are isoenzymes, which differ in subcellular localization, regulation, and possibly function. Arginase I is a cytosolic enzyme, which is expressed mainly in the liver as part of the urea cycle, whereas arginase II is a mitochondrial protein found in a variety of tissues. Antibody to ARG-1 labels hepatocytes in normal tissues and granulocytes in peripheral blood. ARG-1 is a sensitive and specific marker for identification of hepatocellular carcinoma.

Western blot: 2-4 µg/mL

P05089

Human

A portion of amino acids 1-200 from human ARG1 proteinwas used as the immunogen for the Arginase-1 antibody.

ARG1/9446

WB

Protein G affinity

Purified

This Arginase-1 antibody is available for research use only.

Aliquot the Arginase-1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Optimal dilution of the Arginase-1 antibody should be determined by the researcher.

Cytoplasm

Western blot testing of human liver tissue lysate with Arginase 1 antibody (clone ARG1/9446) . Predicted molecular weight ~35 kDa.