ARHGEF12 Antilichaam / LARG

ARHGEF12 is widely expressed in multiple tissue types and has been implicated in various physiological and pathological conditions, such as embryonic development, wound healing, and cancer progression. Alternative splicing of the ARHGEF12 gene gives rise to several isoforms that may vary in function and cellular localization, making precise detection and characterization essential in research. A high-quality ARHGEF12 antibody is a critical tool for investigating the diverse roles of this protein in cellular signaling and disease. The ARHGEF12 antibody can be used in western blotting to evaluate protein levels, in immunofluorescence to assess subcellular localization, and in immunohistochemistry for tissue-specific expression profiling. NSJ Bioreagents offers an extensively validated ARHGEF12 antibody designed to provide specificity and reproducibility across multiple experimental platforms.

Western blot: 0.5-1 µg/mL, Flow cytometry: 1-3 µg/mL, Direct ELISA: 0.1-0.5 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunofluorescence: 5 µg/mL, Immunoprecipitation: 2ug per 500ug of lysate

Q9NZN5

Human, Mouse, Rat

Recombinant human protein (amino acids 10-1426) was used as the immunogen for the ARHGEF12 antibody.

WB, FACS, IHC-P, IF, IP, Direct ELISA

Antigen affinity purified

Antigen affinity purified

Lyophilized from 1X PBS with 2% Trehalose

This ARHGEF12 antibody is available for research use only.

After reconstitution, the ARHGEF12 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Optimal dilution of the ARHGEF12 antibody should be determined by the researcher.

Cytoplasm, cell membrane

IHC staining of FFPE human liver cancer tissue with ARHGEF12 antibody, HRP-labeled secondary and DAB substrate. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.