ARHGEF12 Antilichaam / Rho guanine nucleotide uitwisselingsfactor 12

ELISA: 0.1-0.5 µg/mL, Flow cytometry: 1-3ug/million cells, Immunoprecipitation: 2-4ug/500ug of lysate, Immunofluorescence: 5 µg/mL, Immunohistochemistry: 2-5 µg/mL, Immunocytochemistry: 5 µg/mL, Western Blot: 0.25-0.5 µg/mL

Q9NZN5

Human, Mouse, Rat

E.coli-derived human LARG/ARHGEF12 recombinant protein (Position: D10-E1426) was used as the immunogen for the ARHGEF12 antibody.

ELISA, FACS, IP, IF, IHC, ICC, WB

Immunogen affinity purified

Lyophilized

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

This ARHGEF12 antibody is available for research use only.

After reconstitution, the ARHGEF12 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

Optimal dilution of the ARHGEF12 antibody should be determined by the researcher.

Cytoplasmic

Immunohistochemical staining of ARHGEF12L using anti-ARHGEF12L antibody. ARHGEF12L was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution) . The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-ARHGEF12L antibody overnight at 4oC. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37oC. The tissue section was developed using an HRP secondary and DAB substrate.