ARID1A Antilichaam / AT rijk interactief domein 1A

The SWI-SNF complex is involved in the activation of transcription via the remodeling of nucleosome structure in an ATP-dependent manner. Brm (also designated SNF2a) and Brg-1 (also designated SNF2b) are the ATPase subunits of the mammalian SWI-SNF complex. Brm, Brg-1, Ini1 (integrase interactor 1, also designated SNF5), BAF155 (also designated SRG3) and BAF170 are thought to comprise the functional core of the SWI-SNF complex. Addition of Ini1, BAF155 and BAF170 to Brg-1 appears to increase remodeling activity. Other complex subunits, such as BAF250a (p270 or ARID1A) and BAF250b (ARID1B), are thought to play regulatory roles.

Immunohistochemistry (FFPE) : 1-2 µg/mL

O14497

Human

A portion of amino acids 1800-2000 from human ARID1A protein was used as the immunogen for the ARID1A antibody.

ARID1A/7736

IHC-P

Protein G affinity

Purified

This ARID1A antibody is available for research use only.

Aliquot the ARID1A antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Optimal dilution of the ARID1A antibody should be determined by the researcher.

Nucleus

IHC staining of FFPE human colon tissue with ARID1A antibody (clone ARID1A/7736) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.