ARL1 cDNA

ARL encoded by this gene belongs to the ARL (ADP-ribosylation factor-like) family of proteins, which are structurally related to ADP-ribosylation factors (ARFs) . ARFs, described as activators of cholera toxin (CT) ADP-ribosyltransferase activity, regulate intracellular vesicular membrane trafficking, and stimulate a phospholipase D (PLD) isoform. Although, ARL proteins were initially thought not to activate CT or PLD, later work showed that they are weak stimulators of PLD and CT in a phospholipid dependent manner. Alternative splicing results in multiple transcript variants encoding different isoforms.

ARFL1

12q23.2

Human

PATGen (puc19-derived cloning vector)

ATGGGTGGCTTTTTCTCAAGTATATTTTCCAGTCTGTTTGGAACTCGGGAAATGAGAATTTTAATTTTGGGATTAGATGGAGCAGGAAAAACCACAATTTTGTACAGATTACAAGTGGGAGAAGTTGTTACTACTATACCTACCATTGGATTTAATGTAGAGACGGTGACGTACAAAAACCTTAAATTCCAAGTCTGGGATTTAGGAGGACAGACAAGTATCAGGCCATACTGGAGATGTTACTATTCAAACACAGATGCAGTCATTTATGTAGTAGACAGTTGTGACCGAGACCGAATTGGCATTTCCAAATCAGAGTTAGTTGCCATGTTGGAGGAAGAAGAGCTGAGAAAAGCCATTTTAGTGGTGTTTGCAAATAAACAGGACATGGAACAGGCCATGACTTCCTCAGAGATGGCAAATTCACTTGGGTTACCTGCCTTGAAGGACCGAAAATGGCAGATATTCAAAACGTCAGCAACCAAAGGCACCGGCCTTGATGAGGCAATGGAATGGTTAGTTGAAACATTAAAAAGCAGACAGTAA

ARL1, ARFL1, ARL1, ATGD0135-10 µg, ATGD0135-20 µg, ATGD0135-50 µg, ATGD0135-100 µg, ATGD0135-250 µg, ATGD0135-500 µg, ATGD0135-1 mg, ATGD0135-10, ATGD0135-20, ATGD0135-50, ATGD0135-100, ATGD0135-250, ATGD0135-500, ATGD0135-1

Store the plasmid at -20C.

Lyophilized

Metabolism

603425

NP_001168.1

Human

1. Centrifuge at 7000rpm for 1 minute.; ;2. Carefully open the vial and add 100 µL of sterile water to dissolve the DNA.; ; Each tube contains approximately 10 µg of lyophilized plasmid.

NM_001177.5

546bp

This shuttle vector contains the complete ORF. It is inseted BamH I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector.