ASC Antilichaam

ASC Antibody: Apoptosis is regulated by death domain (DD) and/or caspase recruitment domain (CARD) containing molecules and a caspase family of proteases. CARD containing cell death regulators include RAIDD, RICK, BCL10, Apaf-1, ARC, caspase-9, and caspase-2. A novel CARD domain containing protein was recently identified in human and mouse and designated ASC and TMS1. Ectopic expression of ASC/TMS1 induced apoptosis through activation of caspase-9 and inhibited the survival of human breast cancer cells (3, 4) . Overexpression of ASC/TMS1 induced DNA fragmentation. ASC/TMS1 is expressed in a variety of human and mouse tissues.

29108

Q9ULZ3

BAA87339

Human

2287

Unconjugated

Primary Antibodies

Independent Antibody Validation in Cell lines (Figure 2) shows similar ASC expression profile in human and mouse cell lines detected by two independent anti-ASC antibodies that recognize different epitopes, 2287 against C-terminus domain and 39-001 against full length human recombinant protein. ASC proteins are detected in some of tested cell lines at different expression levels by the two independent antibodies. Independent Antibody Validation in Cell lines2287 39-001

Apoptosis, Inflammasomes

ASC Antibody is affinity chromatography purified via peptide column.

Cat. No. 1209 - HL60 Cell Lysate

1 mg/mL

Predicted species reactivity based on immunogen sequence: Mouse (57%), Rat (57%)

ASC Antibody is supplied in PBS containing 0.02% sodium azide.

None

Blue Ice

ASC antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

IgG

Human ASC has 3 isoforms, including isoform 1 (195aa, 22kD), isoform 2 (176aa, 20kD), and isoform 3 (135aa, 15kD) . Mouse ASC has one isoform (193aa, 22kD) and rat ASC also has one isoform (193aa, 22kD) . 2287 can detect all human isoforms.

PYCARD

Cat. No. 17-009 - HL-60 Cell Slide

Cat. No. 10-901 - Human Spleen Tissue Slide

Cat. No. 1208 – THP-1 Cell Lysate

PYD and CARD domain containing

Homo sapiens

Masumoto et al. J Biol Chem. 1999;274 (48) :33835-8.

Masumoto et al. Exp Cell Res. 2001;262 (2) :128-133.

Conway et al. Cancer Res. 2000;60 (22) :6236-42

McConnell et al. Cancer Res. 2000;60 (22) :6243-7. (WD0102)

15020601

ASC Antibody: ASC, TMS, TMS1, CARD5, TMS-1, ASC, Apoptosis-associated speck-like protein containing a CARD, Caspase recruitment domain-containing protein 5, hASC

ELISA, WB, ICC, IHC-P

10801602

Liquid