ATF4 (fosfo Ser245) Antilichaam

ATF4 encodes a transcription factor that was originally identified as a widely expressed mammalian DNA bindingprotein that could bind a tax-responsive enhancer element in the LTR of HTLV-1. The encoded protein was also isolated and characterized as the cAMP-response element binding protein 2 (CREB-2) . The protein encoded by this gene belongs to a family of DNA-binding proteins that includes the AP-1 family of transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. These transcription factors share a leucine zipper region that is involved in protein-protein interactions, located C-terminal to a stretch of basic amino acids that functions as a DNA binding domain. Two alternative transcripts encoding the same protein have been described. Two pseudogenes are located on the X chromsome at q28 in a region containing a large inverted duplication.

468

P18848

NP_001666.2

Human

ATF4 (Phospho-Ser245) antibody was raised against a peptide sequence around phosphorylation site of serine 245 (N-R-S (p) -L-P) derived from Human ATF4.

Unconjugated

Primary Antibodies

Phospho-Specific

Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

1 mg/mL

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Phospho-Specific

Blue Ice

Store antibody at -20˚ C for up to one year.

45 kDa

This antibody detects endogenous level of ATF4 only when phosphorylated at serine 245.

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

ATF4

Activating transcription factor 4

Homo sapiens

Yang X, et al. (2004) . Cell. 117 (3) : 387-398.

CREB2, TXREB, CREB-2, TAXREB67, EIF4EL1, EIF4F, C/ATF, C/EBP-related ATF, CREB2

IHC, WB

33469976

Liquid