ATG5 Antilichaam

ATG5 Antibody: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1. ATG5, another member of the autophagy protein family, forms a conjugate with ATG12; this conjugate has a ubiquitin-protein ligase (E3) -like activity for protein lipidation in autophagy. This conjugate also associates with innate immune response proteins such as RIG-I and VISA (also known as IPS-1), inhibiting type I interferon production and permitting viral replication in host cells.

9474

A9UGY9

EAW48415

Human, Mouse

5031

Unconjugated

Primary Antibodies

Independent Antibody Validation in Cell lines (Figure 2) shows similar ATG5 expression profile in human and mouse cell lines detected by two independent anti-ATG5 antibodies that recognize different epitopes, 5031 against N-terminus domain and 64-229 against human recombinant protein. ATG5 proteins are detected in all the tested cell lines at different expression levels by the two independent antibodies. Independent Antibody Validation in Cell lines5031

Autophagy

ATG5 Antibody is affinity chromatography purified via peptide column.

Cat. No. 1406 - Mouse Spleen Tissue Lysate

1 mg/mL

Predicted species reactivity based on immunogen sequence: Rat (100%), Bovine: (94%), Pig: (94%)

ATG5 Antibody is supplied in PBS containing 0.02% sodium azide.

None

Blue Ice

ATG5 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

IgY

Three isoforms of ATG5 are known to exist; this ATG5 antibody will only detect the longest isoform.

Human ATG5 has 2 isoforms, including isoform L (275aa, 32.4kD) and isoform S (197aa, 22.9kD. This antibody detects only human isoform L. Mouse ATG5 has 1 isoform (275aa, 32.4kD) . Rat ATG5 has only one isoform identified so far (275aa, 32.4kD) .

ATG5

Cat. No. 10-901 - Human Spleen Tissue Slide

ATG5 autophagy related 5 homolog (S. cerevisiae)

Homo sapiens

Gozuacik and Kimchi. Oncogene 2004; 23:2891-906.

Kisen et al. Carcinogenesis 1993; 14:2501-5.

Kamada et al. J. Cell. Biol. 2000; 150:1507-13.

Hanada et al. J. Biol. Chem.2007; 282:37298-302.

ATG5 Antibody: ASP, APG5, APG5L, hAPG5, APG5-LIKE

ELISA, WB, IHC-P

119568800

Liquid