ATG7 antilichaam

E1-like activating enzyme involved in the 2 ubiquitin-like systems required for cytoplasm to vacuole transport (Cvt) and autophagy. Activates ATG12 for its conjugation with ATG5 as well as the ATG8 family proteins for their conjugation with phosphatidylethanolamine. Both systems are needed for the ATG8 association to Cvt vesicles and autophagosomes membranes. Required for autophagic death induced by caspase-8 inhibition. Required for mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Modulates p53/TP53 activity to regulate cell cycle and survival during metabolic stress. Plays also a key role in the maintenance of axonal homeostasis, the prevention of axonal degeneration, the maintenance of hematopoietic stem cells, the formation of Paneth cell granules, as well as in adipose differentiation.

Ubiquitin-like modifier-activating enzyme ATG7|ATG12-activating enzyme E1 ATG7|Autophagy-related protein 7 (APG7-like, hAGP7) |Ubiquitin-activating enzyme E1-like protein|ATG7|APG7L

10533

O95352

Human, Mouse, Rat

ATG7 autophagy related 7 homolog

ATG7

Immunology, Cardiovascular, Metabolism

Protein A+G purification

WB: 1:1000-1:5000; IHC: 1:50-1:200; IF: 1:20-1:100

≥95% as determined by SDS-PAGE

Liquid

68-78 kDa

4°C with ice bag

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

8H1

ELISA, WB, IHC, IF