ATP5B Antilichaam

ATP5B is a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c) . It is the beta subunit of the catalytic core.

Western blot: 1:1000, Immunohistochemistry (FFPE) : 1:25-1:100, Flow cytometry: 1:25 (1x10e6 cells), Immunofluorescence: 1:25

P06576

Human

A portion of amino acids 135-163 from the human protein were used as the immunogen for the ATP5B antibody.

WB, IHC-P, FACS, IF

Antigen affinity purified

Purified

This ATP5B antibody is available for research use only.

Aliquot the ATP5B antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

In 1X PBS, pH 7.4, with 0.09% sodium azide

The stated application concentrations are suggested starting points. Titration of the ATP5B antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Cytoplasmic

IHC testing of FFPE human small intestine tissue with ATP5B antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.