ATP5F1D Antilichaam / ATP synthase F1 subunit delta

This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c) . This gene encodes the delta subunit of the catalytic core. Alternatively spliced transcript variants encoding the same isoform have been identified.

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunofluorescence: 5 µg/mL, ELISA: 0.1-0.5 µg/mL

P30049

Human, Mouse, Rat

An E.coli-derived human recombinant protein (amino acids R8-E168) was used as the immunogen for the ATP5F1D antibody.

WB, IHC-P, IF, ELISA

Antigen affinity purified

Antigen affinity purified

Lyophilized from 1X PBS with 2% Trehalose

This ATP5F1D antibody is available for research use only.

After reconstitution, the ATP5F1D antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Optimal dilution of the ATP5F1D antibody should be determined by the researcher.

Cytoplasm

Immunofluorescent staining of FFPE human HeLa cells with ATP5F1D antibody (red) and DAPI nuclear stain (blue) . HIER: steam section in pH6 citrate buffer for 20 min.