ATP5MC1/2/3 Antilichaam

The ATP5MC1 gene is one of three human paralogs that encode membrane subunit c of the mitochondrial ATP synthase. It is mapped to 17q21.32. This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8) . This gene is one of three genes that encode subunit c of the proton channel. Each of the three genes have distinct mitochondrial import sequences but encode the identical mature protein. Alternatively spliced transcript variants encoding the same protein have been identified.

Immunohistochemistry (FFPE) : 1-2 µg/mL, Immunofluorescence: 2-4 µg/mL, Flow cytometry: 1-3ug/1x10^6 cells

P05496

Human, Mouse, Rat

Amino acids D62-L113 from the human protein were used as the immunogen for the ATP5MC1/2/3 antibody.

IHC-P, IF, FACS

Affinity purified

Antigen affinity purified

Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

This ATP5MC1/2/3 antibody is available for research use only.

Store the ATP5MC1/2/3 antibody at -20°C.

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Optimal dilution of the ATP5MC1/2/3 antibody should be determined by the researcher.

Mitochondrial

IHC staining of FFPE human ovarian cancer with ATP5MC1/2/3 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.