AurB (fosfo Thr232) Antilichaam

Serine/threonine-protein kinase component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Involved in the bipolar attachment of spindle microtubules to kinetochores and is a key regulator for the onset of cytokinesis during mitosis. Required for central/midzone spindle assembly and cleavage furrow formation. AURKB phosphorylates the CPC complex subunits BIRC5/survivin, CDCA8/borealin and INCENP. Phosphorylation of INCENP leads to increased AURKB activity.

9212

Q96GD4

NP_001243763.1

Human, Mouse, Rat

AurB (phospho Thr232) antibody was raised against a peptide sequence around phosphorylation site of threonine 232 (R-K-T (p) -M-C) derived from Human AurB.

Unconjugated

Primary Antibodies

Phospho-Specific

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

1 mg/mL

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Phospho-Specific

Blue Ice

Store antibody at -20˚ C for up to one year.

39 kDa

IgG

The antibody detects endogenous level of AurB only when phosphorylated at threonine 232.

Western Blot: 1:500~1:1000

AURKB

Aurora kinase B

Homo sapiens

Caldas GV, DeLuca KF, DeLuca JG (2013) J Cell Biol 203, 957-69. Zhang L, et al. (2012) J Biol Chem 287, 34069-77. Ratushny V, et al. (2012) Oncogene 31, 1217-27 .

AIK2, AIM-1, ARK2, AURKB, Aurora-B

WB

317373473

Liquid