B-NGF / Beta-NGF Eiwitten

Nerve growth factor (NGF) is important for the development and maintenance of the sympathetic and sensory nervous systems. NGF protein was identified as a large complex consisting of three non-covalently linked subunits, α, β, and γ, among which, the β subunit, called β-NGF (beta-NGF), was demonstrated to exhibits the growth-stimulating activity of NGF protein. NGFB/beta-NGF gene is a member of the NGF-beta family and encodes a secreted protein that homodimerizes and is incorporated into a larger complex. NGF protein acts via at least two receptors on the surface of cells (TrkA and p75 receptors) to regulate neuronal survival, promote neurite outgrowth, and up-regulate certain neuronal functions such as mediation of pain and inflammation. Also, previous studies indicated that NGF may also have an important role in the regulation of the immune system.

Beta-NGF Protein; HSAN5 Protein; NGFB Protein

NP_002497.2

Human

Human

See Datasheet send with the product

< 1.0 EU per μg of the protein as determined by the LAL method

≥ 95 % as determined by SDS-PAGE, ≥ 90 % as determined by SEC-HPLC, ≥ 90% as determined by SEC-MALS

Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is typically 0.2-2 ng/mL.

118

Lyophilized

40mM His, 40mM Arg, 150mM NaCl, pH 5.5

See Datasheet

30 kDa

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

CHO cells