B-raf Antilichaam

B-raf Antibody: B-raf belongs to the raf/mil family of serine/threonine protein kinases and plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. The Ras/Raf/MEK/ERK and Ras/PI3K/PTEN/Akt pathways interact with each other to regulate growth and in some cases tumorigenesis. Mutations in B-raf have been associated with several cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung, leading to speculation on the possibility of B-raf as a therapeutic target for treating cancers. Mutations in this gene have also been associated with cardiofaciocutaneous syndrome (CFCS), a disease characterized by heart defects, mental retardation and a distinctive facial appearance.

673

P15056

P15056

Human, Mouse, Rat

5777

Unconjugated

Primary Antibodies

Independent Antibody Validation in Cell lines (Figure 2) shows similar B-raf expression profile in human and mouse cell lines detected by two independent anti-B-raf antibodies that recognize different epitopes, 5777 against central domain and 7379 against a slight different central domain. B-raf proteins are detected in the most tested cell lines at different expression levels by the two independent antibodies. Independent Antibody Validation in Cell lines5777 7379

Cancer, Cell Cycle

B-raf Antibody is affinity chromatography purified via peptide column.

Cat. No. 1303 - Human Brain Tissue Lysate

1 mg/mL

Predicted species reactivity based on immunogen sequence: Chicken: (93%)

B-raf Antibody is supplied in PBS containing 0.02% sodium azide.

None

Blue Ice

B-raf antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

IgY

Human B-raf has one isoform (766aa, 84kD) . Mouse B-raf has 2 isoforms, including isoform 1 (804aa, 89kD) and isoform 2 (329aa, 37kD) . Rat B-raf has one isoform (712aa, 80kD) . 5777 can detect in human B-raf and mouse isoform 1.

BRAF

Cat. No. 1210 – 293 Cell Lysate

Cat. No. 1224 – Daudi Cell Lysate

Cat. No. 1204 – K562 Cell Lysate

V-raf murine sarcoma viral oncogene homolog B1

Homo sapiens

McCubrey et al. Biophys. Acta 2007; 1773:1263-84.

Madhunapantula and Robertsonet al. Cancer Res.2008; 68:5-8.

Sarkozy et al. Hum. Mutat.2009; 30:695-702.

B-raf Antibody: NS7, BRAF1, RAFB1, B-RAF1, Proto-oncogene B-Raf

ELISA, WB

50403720

Liquid