B16-F10 Lysaat

B16-F10 cells are derived from mouse skim melanoma. B16 melanoma cell line and its sublines are well-established models for the study of experimental cancer therapies. Melanomas express different tumor-associated Ags, which are potential targets for novel designs of therapeutic cancer vaccines. Many immunotherapeutic protocols have been tested using the B16 cell line. Growth of B16 cells can be controlled in vivo by different classes of specific or nonspecific immune effector cells.

Mouse

WB

2 mg/mL (determined by BCA assay)

B16-F10, ATGL0025-10 µg, ATGL0025-20 µg, ATGL0025-50 µg, ATGL0025-100 µg, ATGL0025-250 µg, ATGL0025-500 µg, ATGL0025-1 mg, ATGL0025-10, ATGL0025-20, ATGL0025-50, ATGL0025-100, ATGL0025-250, ATGL0025-500, ATGL0025-1

Aliquot the lysate into single tube and then storing them -80C or below. Avoid repeated freezing and thawing cycles.

Liquid in. SDS-PAGE Sample Buffer (0.12M Tris-HCl buffer pH6.8, 0.4% SDS, 5% glycerol, 1% beta-mercaptoethanol, 0.02% Bromophenol blue)

Lysates

Mouse

All lanes : B16-F10 Whole Cell Lysate (40ug) ; ; ; ; Lane 1. : Anti-GAPDH antibody (ATGA0181) ; ; Lane 2. : Anti-Beta tubulin antibody (ATGA0196)

Heat lysate to 95°C for 5 minutes and rapidly cooling before use.

Western Blot analyzed by both GAPDH and Beta tubulin antibody