BACE Antilichaam

BACE Antibody: Accumulation of the amyloid-beta (Abeta) plaque in the cerebral cortex is a critical event in the pathogenesis of Alzheimer's disease. Abeta peptide is generated by proteolytic cleavage of the beta-amyloid protein precursor (APP) at beta- and gamma-sites by two proteases. APP is first cleaved by beta-secretase, producing a soluble derivative of the protein and a membrane anchored 99-amino acid carboxy-terminal fragment (C99) . The C99 fragment serves as substrate for gamma-secretase to generate the 4 kDa amyloid-beta peptide, which is deposited in the brains of all suffers of Alzheimer's disease. The long-sought beta-secretase was recently identified by several groups independently and designated beta-site APP cleaving enzyme (BACE) and aspartyl protease 2 (Asp2) . BACE/Asp2 is a novel transmembrane aspartic protease and colocalizes with APP.

23621

P56817

AF190725

Human, Mouse

2253

Unconjugated

Primary Antibodies

Independent Antibody Validation in Cell lines (Figure 2) shows similar BACE expression profile in human cell lines detected by two independent anti-BACE antibodies that recognize different epitopes, 2253 against C-terminus domain and 32-238 against recombinant fragment protein. BACE proteins are detected in all the tested cell lines except K562 at different expression levels by the two independent antibodies. Independent Antibody Validation in Cell lines2253 KO validation (Figure 6,9) : Anti-BACE antibody (2253) specificity was further verified by BACE KO mice (figure6) and KO cell line (figure9) . BACE signal was not detected in BACE KO mice and KO cell line. KO validation2253 KD validation (Figure 7,8,10) : Anti-BACE antibody (2253) specificity was verified by BACE specific siRNA knockdown. BACE signal in mouse brain injected with BACE siRNAs and DRG transfected with BACE siRNAs was disrupted in comparison with control. KD validation2253 Overexpression validation (Figure 6) : Anti-BACE antibody (2253) detected high expression levels of BACE in 293 cells transfected with hBACE or mBACE (figure6) as compared to eGFP transfected cells. Overexpression validation2253

Neuroscience, Cancer

BACE Antibody is affinity chromatography purified via peptide column.

Cat. No. 1303 - Human Brain Tissue Lysate

1 mg/mL

Predicted species reactivity based on immunogen sequence: Guinea pig: (94%), Rat: (94%), Bovine: (94%)

BACE Antibody is supplied in PBS containing 0.02% sodium azide.

None

Blue Ice

BACE antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

IgG

Human BACE has 6 isoforms, including isoform A (501aa, 55.8kD), isoform B (476aa, 52.9kD), isoform C (457aa, 51.1kD), isoform D (432aa, 48.2kD), isoform 5 (401aa, 45kD) and isoform 6 (376aa, 42.2kD) . This antibody detects all human isoforms. Mouse BACE has only 1 isoform (501aa, 55.7kD) . Rat BACE has only one isoform identified so far (501aa, 55.8kD) .

BACE1

Cat. No. 1282 - 3T3/NIH Cell Lysate

Beta-site APP-cleaving enzyme 1

Homo sapiens

Vassar et al. Science 1999;286:735-41

Hussain et al. Mol Cell Neurosci 1999;14:419-27

Yan et al. Nature 1999;402:533-7

Sinha et al. Nature 1999;402:537-40

16136043

18687504

20354142

11863452

19166938

21073551

14507929

12480937

16027115

17912249

18387811

29217476

18349519

20026385

17307738

17877641

20555139

20005283

22551668

BACE Antibody: ASP2, BACE, HSPC104, KIAA1149, Beta-secretase 1, Aspartyl protease 2, ASP2

ELISA, WB, ICC, IHC-P, IF

6118538

Liquid