BAD (fosfo Ser136) Antilichaam

The protein encoded by BAD gene is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin were found to be involved in the regulation of this protein. Alternative splicing of this gene results in two transcript variants which encode the same isoform.

572

Q92934

NP_004313.1

Mouse

BAD (Phospho-Ser136) antibody was raised against a peptide sequence around phosphorylation site of serine136 (S-R-S (p) -A-P) derived from Mouse BAD.

Unconjugated

Primary Antibodies

Phospho-Specific, Apoptosis, Cancer

Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

1 mg/mL

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Phospho-Specific

Blue Ice

Store antibody at -20˚ C for up to one year.

23 kDa

This antibody detects endogenous level of BAD only when phosphorylated at serine136.

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

BAD

BCL2-associated agonist of cell death

Murine

Wang XQ, et al. (2001) . J Biol Chem. 276 (48) : 44504-44511. Lee YI, et al. (2001) . J Biol Chem. 276 (20) : 16969-16977. Maiti D, et al. (2001) . J Biol Chem. 276 (1) : 329-333.

BBC2, BCL2L8, BBC6

WB, IHC

10835069

Liquid