BaF3 Lysaat

BaF3 cell line was immortalized murine bone marrow-derived pro-B-cell line. BaF3 cells are depends IL3 for growth and proliferation. When cultured in the presence of IL3, or other growth-promoting factors, BaF3 cells are highly resistant to X-irradiation and the cytotoxic drugs.

Mouse

WB

2 mg/mL (determined by BCA assay)

BaF3, ATGL0034-10 µg, ATGL0034-20 µg, ATGL0034-50 µg, ATGL0034-100 µg, ATGL0034-250 µg, ATGL0034-500 µg, ATGL0034-1 mg, ATGL0034-10, ATGL0034-20, ATGL0034-50, ATGL0034-100, ATGL0034-250, ATGL0034-500, ATGL0034-1

Aliquot the lysate into single tube and then storing them -80C or below. Avoid repeated freezing and thawing cycles.

Liquid in. SDS-PAGE Sample Buffer (0.12M Tris-HCl buffer pH6.8, 0.4% SDS, 5% glycerol, 1% beta-mercaptoethanol, 0.02% Bromophenol blue)

Lysates

Mouse

All lanes : BaF3 Whole Cell Lysate (40ug) ; ; ; ; Lane 1. : Anti-GAPDH antibody (ATGA0181) ; ; Lane 2. : Anti-Beta tubulin antibody (ATGA0196) ;

Heat lysate to 95°C for 5 minutes and rapidly cooling before use.

Western Blot analyzed by both GAPDH and Beta tubulin antibody