BAFF Antilichaam [Buffy-2]

10673

Q9Y275

Human, Mouse

BAFF (monoclonal Buffy-2) antibody was raised against recombinant human soluble BAFF (aa. 83-285) .

Buffy-2

Unconjugated

Primary Antibodies

Chemokines & Cytokines, Cancer

Anti BAFF (monoclonal Buffy-2) antibody was purified to ≥95% in SDS-PAGE.

Batch dependent

PBS containing 0.02% sodium azide

None

Blue Ice

BAFF antibody can be stored at 4˚ C, stable for one year.

IgM

BAFF antibody can be used to recognize membrane-bound and soluble human and mouse (weak) BAFF by Flow Cytometry, Immunohistochemistry (frozen sections, paraffin sections) and Western Blot. Method: HEK 293T cells (5 x 105) were mock transfected (thin line) or transfected with an expression plasmid enabling surface expression of full length hBAFF (thick line) . Cells were incubated on ice for 30min in 50 μ l FACS buffer (PBS, 5% fetal calf serum, 0.02% azide) containing 1 μ g/mL of MAb to BAFF (Buffy-2) (FITC) . After washing, cells were analyzed by flow cytometry. Method: Tissues are in paraffin sections. Sections are de-waxed and rehydrated, then cooked. Sections are stained in wet boxes, rehydrated and finally prepared as coverslip slides using Eukitt solution. Please inquire for complete protocol..

Tumor necrosis factor (ligand) superfamily, member 13b

Homo sapiens

Association of BAFF/BLyS overexpression and altered B cell differentiation with Sjogren's syndrome: J. Groom, et al.; J. Clin. Invest. 109, 59 (2002) .

Expression of BCMA, TACI, and BAFF-R in multiple myeloma: a mechanism for growth and survival: A. J. Novak, et al.; Blood 103, 689 (2004) .

Lymphoma B cells evade apoptosis through the TNF family members BAFF/BLyS and APRIL: B. He, et al.; J. Immunol. 172, 3268 (2004) .

BAFF production by antigen-presenting cells provides T cell co-stimulation: B. Huard, et al.; Int. Immunol. 16, 467 (2004) .

WB, IHC, Flow

Liquid